General Cell Lines and Cell Cultures
ATCC Cryptococcus neoformans; WM628
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC Mycoplasma hominis; Strain LBD-4
Mycoplasma hominis strain [LBD-4] was isolated from human blood. This titered product is useful for assay development and quality control.
ATCC CCD-1079Sk; Foreskin; Human (Homo Sapiens)
CCD-1079Sk are fibroblasts that were isolated from the skin of a normal male neonate donor. It can be used in cancer research or to derive induced pluripotent stem cells.
Eurofins Discoverx ChemiBrite TP Prostanoid Receptor Stable Cell Line, 2 vials. (HTS081L)
The ChemiBrite TP Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin H2, thromboxane A2) based activation of TBXA2R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TBXA2R alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of TBXA2R, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen AT1 Angiotesin Receptor Stable Cell Line, 2 vials. (HTS064C)
The ChemiScreen AT1 Angiotensin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. Angiotensin-II) based activation of AGTR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AGTR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of AGTR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CRTH2/DP2 Prostanoid Receptor Membrane Preparation, 1 vial. (HTS031M)
The ChemiScreen CRTH2/DP2 Prostanoid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of PTGDR2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC SHM-D33; Somatic Cell Hybrid; Human (Homo sapiens)/Mouse (Mus musculus)
SHM-D33 is a somatic B lymphoblast cell hybridoma that was isolated from the heteromyeloma of a mouse. This cell line was deposited by HS Kaplan and can be used in immunology research.
ATCC HCT116-SLC15A4-KO-c10; Colorectal carcinoma; Human (Homo sapiens)
The HCT116-SLC15A4-KO-c10 cell line contains a -7 deletion in SLC15A4 gene and combined with the parental cell line and other HCT116 derivatives can be used in cancer drug screening.
ATCC Yarrowia lipolytica; PO1f
An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.
ATCC Rhizobium rhizogenes
Rhizobium rhizogenes strain [CIP 104786] is a whole-genome sequenced strain that produces isoprene. This bacterium has applications in agricultural research.
ATCC Malassezia globosa; CBS 7966
An ampoule containing viable cells (may include spores and mycelium) suspended in cryoprotectant.
Eurofins Discoverx ChemiScreen β2 Adrenoceptor Receptor Stable Cell Line, 2 vials. (HTS073C)
The ChemiScreen β2 Adrenoceptor Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRB2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRB2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of ADRB2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen V1A Vasopressin Receptor Membrane Preparation, 1 vial. (HTS059M)
The ChemiScreen V1A Vasopressin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of AVPR1A surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.